SEPARA Syringeless filter is one step sample preparation device that integrates a device auto-sampler, filtration, membrane, plug and cap/septa. The device consists of two parts: an internal vial with a membrane chamber and an external vial to be filled with the filtrate. The internal vial has a sealed membrane and a cap with pre-cut septa. The liquid is placed in the external vial and the SEPARA is ready to be loaded in the auto-sampler.